1. Add 2ml of water in the test vial.
2. Add 5 drops of the Cu reagent and swirl gently for 5 seconds.
3. Allow it to stand for 10 minutes. Some copper medications might use strongly bound copper and would require a waiting time of 20 minutes. In case of doubt compare the value after 10 and 20 minutes of waiting. The highest value should be noted.
4. To obtain the copper concentration hold the test vial in front of you. Place the white part of the color chart firmly against the side of the test vial and look from the opposite side.
A blue tinge lighter than the 0.1ppm value corresponds to approximately 0.05ppm.
A color in between two color bars indicates a copper value in between those two values.